Cristiano Ronaldo scored twice late to rescue a 2-1 win for Juventus at Udinese on Sunday hours after the Turin giants lost their Serie A crown to Inter Milan.
Inter broke Juve's nine-year streak of domestic dominance by clinching their 19th 'Scudetto' after nearest rivals Atalanta drew 1-1 at Sassuolo to leave the 'Nerazzurri' 13 points clear with four games remaining.
Antonio Conte's side had needed just one point to take the title regardless of how Atalanta fared after their 2-0 win at Crotone on Saturday, and have denied Juve a 10th consecutive league triumph.
Juve's win moves them up to third as they target a Champions League spot, but are equal on 69 points with second-placed Atalanta and AC Milan in fourth.
Napoli are two points behind in fifth after a 1-1 draw against Cagliari while Lazio are still in the running, five points off a top-four finish with a game in hand after an exciting 4-3 win over Genoa.
"The nine years are over, we will no longer have the Scudetto attached to the shirt, but we will start again with the same desire as before," said Juve coach Andrea Pirlo.
"We earned this win with effort and pride, and it's a very important one given the other results this afternoon.
"We lost points against teams that we should have beaten, sometimes naively, we let Inter escape and now we are focused on reaching the Champions League."
Ronaldo's brace takes his Serie A-leading tally to 27 goals, six more than Inter's Romelu Lukaku.